Abstract: With the increase of magnetic mineral content in the raw ore of Anqian mine, there are many problems such as low grade of gravity separation concentrate, difficulty in improving flotation concentrates quality, and serious iron loss of flotation tailings in the current joint beneficiation process. In order to solve these problems, an experimental research of process optimization was carried out based on the Anqian iron ore, including grinding, two stages of low intensity magnetic separation, high intensity magnetic separation, and reverse flotation. The results showed that under the condition of the optimal grinding fineness of −0.043 mm with a content of 80%, the magnetic induction intensity of both two stages low intensity magnetic separation of 0.1 T, and the magnetic induction intensity of high intensity magnetic separation of 0.7 T, the mixed magnetic concentrates consisted of the second stage low intensity magnetic separation tailings and high intensity magnetic separation concentrates was obtained, which was used as the feed of reverse flotation. Furthermore, the optimal reagent regime for reverse flotation was determined as slurry temperature of 35±1 ℃, pH of 11.5, starch dosage of 800 g/t, CaO dosage of 1000 g/t, and collector (TD−Ⅱ) dosages of 1000 g/t and 450 g/t for rough and cleaning, respectively. Under the condition of the above optimal reagent regime, the technical indicators for flotation concentrate of a TFe grade of 67.16% and a recovery of 93.99% calculated by flotation feed were obtained through one rough, one cleaning, and three scavenging in reverse flotation closed circuit experiments. Two stages low intensity magnetic separation—high intensity magnetic separation—reverse flotation can obtain the final iron concentrate with yield of 36.94%, iron grade of 68.80% and iron recovery of 88.19%. FTIR analysis showed that starch can selectively adsorbed on the surface of hematite through physical adsorption, which inhibited the adsorption of collectors on the surface of hematite.