Abstract: The main reason for the difficulty to separate different minerals in complex ore system is that the dissemination size of useful mineral is too small, and the intergrowth relationship between useful minerals and gangue minerals is too complex, so ore must first be finely ground to realize dissociation between the minerals, which, however, results in mutual covering between minerals and sliming phenomenon of ores, and then the interactive effects of mineral flotation happen and lead to the mineral separation being very difficult. The interactive effects of mineral flotation mean the influence of the adsorption and surface conversion of more than two minerals on flotation separation in the complex ore flotation system. In order to achieve selective separation of useful minerals in complex minerals system, the interaction laws between minerals must first be found out, and then the methods must be sought to eliminate the interactive effects among minerals; and ultimately to improve the separation selectivity of minerals. Recent research achievements about flotation interactive effect law of complex iron ore, magnesite ore and tungsten ore system were described. The crystals chemistry and surface physical and chemical mechanism of flotation interactive effect of various minerals in different types of complex ores were revealed. Methods of weakening or promoting the interactive effect among minerals were put forward. Then a theoretical system of flotation interactive effects for complex iron ore, magnesite ore and scheelite ore were established.