Abstract: Research in process mineralogy and comprehensive mineral processing experiments were conducted in order to rationally develop and utilize a gold-bearing copper sulfide ore. It showed that the grade of the main valuable element copper in the ore was 0.57%, and that of the associated element gold was 1.56 g/t. Copper mainly existed in the form of chalcopyrite, and gold mainly exists in the form of native gold and electrum, the carrier minerals of gold were mostly pyrite and chalcopyrite. The process of copper gold sulfur mixed flotation, copper sulfur separation, sulfur concentrate regrinding, and gold sulfur separation was used with YZ-05 as the collector. Copper concentrate with Cu grade of 19.57% and recovery of 88.7%, Au grade of 36.93 g/t and recovery of 65.5%, Ag grade of 61.00 g/t and recovery of 46.70% was obtained. In the gold concentrate, Au grade was 42.27 g/t, and the recovery was 21.1%. The total recovery of gold was 86.6%. In the sulfur concentrate, S grade was 48.24% and the recovery was 69.70%. The study provided a technical basis for the comprehensive recovery and utilization of such ore.