Abstract: This study was analyzing the beneficiation wastewater of Fankou lead-zinc mine and meeting the technical upgrade requirements, the research on the treatment of wastewater with the technology of "initial oxidation+pretreatment+biochemical treatment" was carried out to explore the wastewater with different ozone, catalytic oxidation time and different hydraulic retention time. Moreover, the influence of wastewater reuse after treatment on mineral processing index was investigated, and the feasibility of the process was evaluated. The results show that O₃ was used to directly or indirectly oxidize the residual organic chemicals in the wastewater in the initial oxidation, ; the co-pretreatment removes Ca²⁺ and heavy metals in the wastewater; the subsequent biochemical treatment further improves the quality of the water. In addition, the ozone concentration is 60 mg/L, the catalytic oxidation time is 20 min, and the hydraulic retention time is 16.71 h, which can reduce the wastewater COD concentration to 34.32 mg/L, and the removal rate is 91.9%. Further research shows that the increase in hydraulic retention time and ozone concentration significantly improves the wastewater treatment effect. Reusing the treated wastewater for ore beneficiation can effectively eliminate the influence of reused water on the instability factors of ore beneficiation, and it is close to the beneficiation index of clean water. Under stable operation, this process can not only realize the efficient treatment and reuse of mineral processing wastewater, but also has a lower processing cost of 2.65 ￥/m³, which can provide usable technical experience for the reuse of wastewater from domestic lead-zinc processing plants.