Abstract: The iron content of an iron ore from Anshan is 32.19%. The iron minerals are mainly magnetite and hematite. The gangue minerals mainly include quartz. For this ore, the magnetic separation-reverse flotation tests were conducted for determining process parameters reasonably and guiding development and utilization of such ore. The test results indicated that the mixed magnetic concentrate was obtained with the grinding fineness under 0.045 mm of 85%, weak magnetic separation magnetic field magnetic induction intensity of 0.1 T, strong magnetic separation background magnetic induction intensity of 0.5 T. Then the process of one roughing, one cleaning and three scavenging flotation was adopted. The final iron concentrate with the grade of 69.97% and the recovery of 80.64% could be obtained under the conditions of the starch dosage of 320 g/t, the calcium oxide dosage of 500 g/t and the total oleate collector dosage of 135 g/t (90 g/t in roughing, 45 g/t in cleaning) in the reverse flotation.