Abstract: O-isopropyl-N, N-diethyl thionocarbamate (XBE) is a new collector developed by the research group, which has good flotation separation effect for copper and sulfur. In order to further study the flotation performance of XBE, the effects of stirring time, reagent concentration, pulp pH and temperature on adsorption capacity of XBE onto chalcopyrite surfaces were studied. The adsorption kinetics and thermodynamics were investigated. The results show that adsorption capacity of XBE on chalcopyrite surfaces didn't change basically when the pH was between 7 and 11. After stirring for 25 minutes, adsorption equilibrium of XBE on chalcopyrite surfaces was reached, and adsorption process conformed to the second-order reaction kinetic equation. The adsorption capacity of XBE on chalcopyrite surfaces increased with the increase of reagent concentration and temperature. The adsorption of XBE on chalcopyrite surfaces conformed to Freundlich multilayer adsorption model.