Abstract: The flotation separation of tungsten ore with 0.55% Mo, 0.79% Bi, 0.66% Cu, 2.25% Zn, 15.95% S, 2.58% As and 35.84% WO₃, which was obtained from trunk flowsheet with gravity concentration of a tungsten concentrator, was studied. According to the ore property, The sulfur-arsenic mixed flotation process was adopted. An efficient activator BK546B as a substitute for conventional sulfuric acid was used not only to improve the removal of sulfur and arsenic in tungsten concentrates, more important to improve the inconvenience of operation and unhealthy working environment caused by using sulfuric acid; the combined effective collector AT608A and butyl xanthate were used to remove sulfur and arsenic minerals from tungsten concentrates, and reduce the contents of sulfur and arsenic in tungsten concentrates and improve the quality of the tungsten concentrates. The results of the closed circuit test show that a tungsten concentrate can be obtained with WO₃ grade 55.64%, S grade 0.38%, As grade 0.088% and WO₃ recovery rate of 99.34%, and 0.66% of WO₃ content and 0.66% of WO₃ loss in the sulfur and arsenic concentrates are achieved separately. It can be achieved the effective removal of the sulfur and arsenic minerals from the tungsten ore by using the new process studied, and solve the difficulty of production and management.