Abstract: A refractory iron-copper ore in Qinghai Province, with characteristics of complex mineral composition and complex dissemination, has a higher content of mafic silicate minerals, such as serpentine, olivine, and chlorite. According to easily slimezation characteristics of mafic silicate minerals, middlings on the first stage were separated further, greatly reducing the circulating load of middlings, weakening circulative accumulation of mud minerals and improving the separation index. Comprehensive recovery of copper and sulfur was carried out using a "bulk flotation, copper-sulphur separation" process. A Copper concentrate with a Cu grade of 16.39% and a sulfur concentrate with an S grade of 31.70% were obtained from the closed circuit test, in which the Cu recovery was 73.04% and the sulfur recovery was 58.32% respectively.